Abstract:In this study，the extraction process of total flavonoids from perilla leaf was studied by ultrasonicmicrowave synergistic extraction technique and their antioxidant activity was studied.Through the single factor test and response surface optimization test，the effects of the four factors of material-liquid ratio，ethanol concentration，extraction time and microwave power on the extraction amount of perilla leaf flavonoids were explored.Extract process parameters and explore the protective effect of perilla leaf flavonoids on oxidative damage of RAW264.7 cells induced by H2O2.The results showed that the optimal extraction process parameters of perilla leaf flavonoids were as follows：material-liquid ratio 1 ∶17（g/mL），ethanol concentration 62%，extraction time 20 min and microwave power 300 W，under this condition，the extraction amount of flavonoids was 8.33 mg/g.In addition，the concentration of perilla leaf flavonoids in the range of 100 μg/mL-400 μg/mL could significantly alleviated the inhibitory effect of H2O2on the proliferation of macrophages and increase the activity of intracellular antioxidant enzymes.It showed that it had good antioxidant activity and can be considered as a new type of natural antioxidant.