Abstract:The aloe gel polysaccharides was extracted from aloe vera gel by different sequential combinations of ultrasonic treatment and multi-enzyme hydrolysis.Experimental design was based on the investigation of collaborative way of ultrasound and multi-enzyme，the effect of the combination for extraction of aloe gel polysaccharides such as ratio of multi-enzyme and extraction conditions on the extraction yield of fresh aloe gel.Response surface methodology was applied to optimize the parameters of the process，so as to determine the best extraction conditions and achieve the purpose of improving the extraction yield of the polysaccharide from aloe vera gel.The results showed that simultaneous treatment was the best combination for extraction of aloe gel polysaccharides，pectinase addition of 1.0%，cellulase addition of 1.5%，and neutral protease addition of 1.0%were the best ratio of multi-enzyme.On these basis，the results indicated that the optimum extraction conditions of the aloe vera gel polysaccharides were as follows：ultrasonic temperature 51 ℃，ultrasonic extraction time 52 min，pH value 4.2.