Abstract:Corn flour was used as raw material to produce glucose，the liquefaction by high temperature enzymatic method and the glucoamylase was added for saccharification and the reducing sugar value（DE value）and glucose mass fraction （DX value） were used as evaluation indexes to investigate the effects of saccharification temperature，saccharification time，pH value and glucoamylase on the saccharification effect.Based on the screening of single-factor experiment design，the saccharification parameters were optimized by orthogonal test method.The results showed that the optimal saccharification conditions were as follows saccharification temperature of 60 ℃ ，saccharification time of 12 hours，pH value 5.3 and glucoamylase amount of 45 U/g.Under these conditions，the DE value was 98.24%and the DX value was 98.16%.