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食品研究与开发:2024,45(7):125-132
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超微粉碎辅助酶解法制备海参冻干粉的生物活性分析
(1.浙江工商大学 食品与生物工程学院,浙江 杭州 310018;2.浙江工商大学 东海食品台州研究院,浙江 台州 318000;3.青岛纽萃生生物健康科技有限公司,山东 青岛 266071;4.大连工业大学 食品学院,辽宁 大连 116034;5.国家海洋食品工程技术研究中心,辽宁 大连 116034)
Bioactivity of Sea Cucumber Freeze-Dried Powder Prepared by Vibration-Assisted Milling Combined with Enzymatic Hydrolysis
(1.School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,Zhejiang,China;2.Donghai Food Research Institute(Taizhou),Zhejiang Gongshang University,Taizhou 318000,Zhejiang,China;3.Qingdao Niucuisheng Biotechnology Co.,Ltd.,Qingdao 266071,Shandong,China;4.School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,Liaoning,China;5.National Engineering Research Center of Seafood,Dalian 116034,Liaoning,China)
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投稿时间:2023-07-17    
中文摘要: 以超微粉碎辅助酶解法制备的海参(Cucumaria frondosa)冻干粉为研究对象,通过DPPH 自由基清除率、羟基自由基清除率和还原力、HeLa 细胞增殖抑制率、小鼠转棒实验及血乳酸(lactic acid,LA)含量和环磷酰胺(cyclophosphamide,CY)诱导的免疫缺陷小鼠实验及免疫球蛋白G(immunoglobulin G,IgG)含量,分别研究海参冻干粉的抗氧化能力、抗肿瘤、抗疲劳和提高免疫力的能力。结果表明,DPPH 自由基清除率的IC50 为12.46 mg/mL,羟基自由基清除率的IC50 为15.22 mg/mL,还原力为0.35,表明海参冻干粉具有抗氧化能力。海参冻干粉对HeLa 细胞增殖抑制率的IC50 为556.2 μg/mL,表现出抗肿瘤能力。通过灌胃海参冻干粉,小鼠转棒运动时间随着剂量升高而延长,运动后高剂量组的LA 含量(19.15 mmol/L)较低,说明小鼠运动耐力提高,且CY 诱导的免疫缺陷小鼠降低了体质量的下降速率,高剂量组IgG 含量恢复到11.98 mg/mL。综上,海参冻干粉具有抗氧化、抗肿瘤抗疲劳和提高免疫力的多功能活性潜力。
Abstract:Sea cucumber freeze-dried powder was prepared by vibration-assisted milling combined with enzymatic hydrolysis.The antioxidant activity of the sea cucumber freeze-dried powder was evaluated based on the DPPH and hydroxyl radical scavenging rates and reducing power.The anti-tumor activity was examined based on the proliferation inhibition rate of HeLa cells.The rotarod test of mice and the blood lactic acid(LA)content were employed to measure the anti-fatigue activity.The mouse model of cyclophosphamide(CY)-induced immune deficiency and the immunoglobulin G(IgG)content were employed to assess the immunity-enhancing activity of the powder.The prepared sea cucumber freeze-dried powder showed the IC50 of 12.46 mg/mL and 15.22 mg/mL for scavenging DPPH and hydroxyl free radicals,respectively,and the reducing power of 0.35,which indicated that the sea cucumber freeze-dried powder possessed antioxidant activity.The sea cucumber freeze-dried powder showed the IC50 of 556.2 μg/mL on the proliferation of HeLa cells,demonstrating anti-tumor activity.The administration of sea cucumber freeze-dried powder prolonged the duration of rotarod exercise of mice in a dose-dependent manner,and the LA content(19.15 mmol/L)in the high-dose group piled up less after exercise,which suggested that the powder improved the exercise endurance of mice.Furthermore,the sea cucumber freeze-dried powder slowed down the body weight loss in the mouse model of CY-induced immune deficiency in a dose-dependent manner,and the IgG content in the high-dose group was restored to 11.98 mg/mL.In conclusion,sea cucumber freeze-dried powder has antioxidant,anti-tumor,anti-fatigue,and immunity-enhancing activities.
文章编号:202407018     中图分类号:    文献标志码:
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