Abstract:A yellow pigment-producing strain BX-1 isolated from reed root soil was isolated and identified，and the culture conditions of yellow pigment produced by BX-1 strain were optimized.By using morphological observation，molecular biology identification method，and single factor and orthogonal experimental designs，the strain BX-1 was Gram-positive bacterium and Glutamicibacter halophytocola.The strain produced intracellular yellow pigment，which was soluble in methanol and had absorption peaks at 411，438 nm and 467 nm.The optimum solid-state culture conditions of the strain BX-1 were as follows.The colony was cultured at 20℃for 240 h，and the solid culture medium was composed of 3% sodium glutamate，3% soybean meal powder，0.5% yeast extract powder，1% sodium chloride，0.25% potassium dihydrogen phosphate，and 0.1% magnesium sulfate，with the initial pH7.0.