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食品研究与开发:2022,43(8):98-105
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超声辅助低共熔溶剂提取玫瑰多酚及其抗氧化活性
(1.天津科技大学食品营养与安全国家重点实验室,天津 300457;2.河北科技大学食品与生物学院,河北 石家庄 050018)
Ultrasonic-assisted Extraction of Rose Polyphenols by Deep Eutectic Solvent and Antioxidant Activity
(1.State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China;2.College of Food and Biology,Hebei University of Science and Technology,Shijiazhuang 050018,Hebei,China)
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投稿时间:2021-07-31    
中文摘要: 玫瑰是一种药食同源的花卉,含有多种活性物质,具有良好的保健价值。以重瓣玫瑰“丰花1号”为原料,采用超声辅助低共熔溶剂(deepeutecticsolvent,DES)法提取玫瑰多酚,并与传统乙醇提取方法进行比较。单因素试验得到的DES法提取玫瑰多酚的最佳工艺条件:含水量为30%的氯化胆碱-乳酸(摩尔比1∶2)为最佳提取剂,料液比1∶40(g/mL)、超声时间10 min、超声功率400 W,超声温度50℃、提取2次,在此条件下多酚提取量为(136.20±1.23)mg/g。与乙醇提取法相比,DES法的多酚提取量提高了24%。抗氧化试验结果表明,玫瑰多酚总还原能力、DPPH自由基清除能力与维生素C相近,而ABTS+自由基清除能力高于维生素C,说明玫瑰多酚具有较强的抗氧化活性。
中文关键词: 玫瑰  多酚  超声辅助  低共熔溶剂  提取  抗氧化性
Abstract:A rose is a flowering plant with medical and nutritional values.It contains a variety of active substances and has beneficial health care properties.In this study,the rose polyphenols were extracted using deep eutectic solvent(DES)with ultrasonic-assisted technology from the double rose'Fenghua No.1'.The traditional ethanol extraction method was also applied to extract rose polyphenols in comparison.The optimal extraction conditions of rose polyphenols by DES method were as follows:choline chloride lactic acid(molar ratio 1∶2)with 30% water content was the best extraction agent,the solid-liquid ratio was1∶40(g/mL),ultrasonic time was10 min,ultrasonic power was 400 W,ultrasonic temperature was 50℃,and extractions were completed two times.Under these conditions,the amount of polyphenols extracted was(136.20±1.23)mg/g.Compared with the ethanol extraction method,the number of polyphenols extracted by DES was increased by 24%.Antioxidant test results showed that the total reducing power and DPPH free radical scavenging ability of the rose polyphenols were similar to VC.Furthermore,ABTS+free radical scavenging capacity was higher than that of VC.These results indicate that rose polyphenols have strong antioxidant activity in vitro.
文章编号:202208013     中图分类号:    文献标志码:
基金项目:天津市科技计划项目(20ZYJDJC00080)
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