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食品研究与开发:2021,42(15):145-151
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藜麦甾醇提取工艺优化及脂肪酸组成分析
(青海大学农牧学院,青海 西宁 810016)
Optimization of Extraction Technology for Quinoa Sterols and Analysis of Fatty Acid Composition
(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China)
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投稿时间:2021-03-19    
中文摘要: 以藜麦为原料,藜麦甾醇得率为考察指标,利用超临界CO2-皂化法萃取藜麦甾醇,通过单因素和正交试验确定最佳提取工艺条件,利用气相色谱串联质谱法(gas chromatography-mass spectrometer,GC-MS)对藜麦油脂进行成分分析。结果表明,提取藜麦甾醇的最佳条件为:萃取压力25 MPa、萃取温度50℃、萃取时间2.0 h,在此条件下,藜麦甾醇的得率可达20.20 mg/g。从藜麦油脂中共分离鉴定出29种脂肪酸,其中主要的脂肪酸为亚油酸、亚麻酸和油酸,不饱和脂肪酸的含量为74.59%,超长链脂肪酸含量高达27.66%,是其它品种藜麦含量的2倍;支链脂肪酸的含量达0.63%。该研究为藜麦的深度开发和质量评价提供理论依据。
中文关键词: 藜麦  甾醇  提取  超临界CO2-皂化法  脂肪酸
Abstract:Using quinoa as a raw material and the quinoa sterol yield as a test index,quinoa sterols were extracted by a supercritical CO2-saponification method involving a single factor and an orthogonal experiment,and the components of quinoa oil were analyzed by gas chromatography-mass spectrometry.The results demonstrate that the optimal conditions were extraction pressure of 25 MPa,extraction temperature of 50 ℃,and extraction time of 2.0 h.Under the optimized conditions,the quinoa oil yield was 20.20 mg/g.A total of 29 fatty acids were isolated from the quinoa oil and identified,with the major components being:linoleic acid,linolenic acid,and oleic acid.The unsaturated fatty acid content was 74.59%,while very long-chain fatty acids accounted for 27.66%,which was twice that of other quinoa varieties;branched-chain fatty acids comprised 0.63%.This study provides a theoretical basis for the further development and quality evaluation of quinoa.
文章编号:202115023     中图分类号:    文献标志码:
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