Abstract:Magnetic molecularly imprinted polymers nanoparticles （MMIPs NPs）with specific identification toward norfloxacin （NOR）were prepared using Fe3O4@NH2NPs as carrier，NOR as template molecule，dopamine（DA）as functional monomer and crosslinking agent.The synthesis condition of MMIPs NPs were optimized，and the adsorption ability of MMIPs NPs toward NOR were studied，which provided a technique for the rapid and efficient enrichment and separation of NOR residues in the food sample.The results showed that the obtained MMIPs NPs displayed the highest adsorption ability for NOR when the volume ratio of ethanolwater （solvent）was 1 ∶1，the addition of Fe3O4@NH2NPs，NOR and DA were 500 mg，15 mg and 134 mg respectively，and the volume ratio of the ethanol-acetic acid （elution solution）was 97 ∶3.The adsorption efficiency reached 94.61% for 1 mL NOR of 0.02 mg/mL when 4 mg MMIPs NPs were used and adsorption time was 60 min.The adsorption capacity of MMIPs NPs toward NOR was 2.18 times than that of magnetic nonmolecular imprinted polymers nanoparticles （MNIPs NPs）.The adsorption efficiency of MMIPs NPs reached 99.17% for 1 mL NOR of 0.1 mg/mL when 15 mg MMIPs NPs were used and adsorption time was 60 min with relative standard deviation of 1.03%.