Abstract:The high performance liquid chromatography （HPLC）was used for establishing a method for the simultaneous determination of quinic acid，malic acid，lactic acid and citric acid in kiwi wine.Chromatographic conditions：mobile phase was 0.01 mol/L potassium dihydrogen phosphate solution，pH value was 2.90，column was InertSustainSwift C18（250 mm×4.6 mm，5 μm），flow rate was 1.00 mL/min，column temperature was 30 ℃，injection volume was 20 μL，and detection wavelength was 215 nm.Under these conditions，the relative standard deviation of precision test of each organic acid was less than 0.10%，the relative standard deviation of reproducibility of each organic acid was less than 1.00 %，the detection limit of 4 kinds of organic acid were among 4.50 mg/L and 6.60 mg/L，the correlation coefficient of each standard curve was above 0.999 0，and the recoveries of the samples were among 99.25 % and 100.16 %.Using this method，organic acid analysis of 10 pieces of kiwi wine revealed that tonic acid，malic acid and citric acid were the main organic acids in kiwi wine，with contents of 7.83 mg/mL-8.60 mg/mL，7.17 mg/mL-7.66 mg/mL and 4.53 mg/mL-6.09 mg/mL respectively.