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投稿时间：2018-09-26
投稿时间：2018-09-26
中文摘要: 为优化江永香菇多糖提取工艺，采用超声波细胞破碎辅以热水浸提，通过单因素试验考察超声时间、超声功率、料液比、浸提时间、浸提温度5 个因素对香菇多糖得率的影响，以香菇多糖得率为响应值，采用响应面设计优化工艺，同时对提取的香菇多糖进行抗氧化活性研究。结果表明，提取的最佳工艺条件为：超声时间6 min、浸提温度78 ℃、浸提时间51 min。在此条件下，江永香菇多糖的得率可达到29.71%。超声波细胞破碎法辅以热水浸提得到的江永香菇多糖体外清除DPPH 自由基能力的IC50值为0.35 mg/mL，清除ABTS+自由基能力的IC50值为1.76 mg/mL，相同浓度下，其DPPH 自由基清除能力和ABTS+自由基清除能力均高于热水回流法提取得到的江永香菇多糖。
Abstract:To optimize extraction process，polysaccharide from Lentinus edodes from Jiangyong was extracted with the ultrasonic-assisted hot water extraction by cell disruption method. Through single factor experiment，the effect of ultrasonic time，solid-liquid ratio，extracting time and the extraction temperature on the yield of lentinan was examined.Response surface design experiment was carried out.Besides，the antioxidant activity invitro of polysaccharide from Lentinus edodes from Jiangyong was evaluated. Experimental results showed that the extracting conditions were ultrasonic time 6 min，temperature 78 ℃，extraction time 51 min.The extraction yield of lentinan was 29.71 %. The IC50 of DPPH free radical clearance ratio and ABTS+free radical clearance ratio of polysaccharide from Lentinus edodes from Jiangyong with the ultrasonic-assisted hot water extraction by cell disruption method were 0.35 mg/mL and 1.76 mg/mL，respectively. The DPPH free radical clearance ratio and ABTS + free radical clearance ratio of polysaccharide from Lentinus edodes from Jiangyong were more effective than those of polysaccharide from Lentinus edodes from Jiangyong with hot water extraction at the same concentration.
keywords: polysaccharide from Lentinus edodes cell disruption response surface method antioxidant research
文章编号：201908021 中图分类号： 文献标志码：
基金项目:湖南省自然科学基金项目（2018JJ3196）；湖南省高校科技创新团队支持计划资助（2012-318）；湖南科技学院湘南优势植物资源综合利用湖南省重点实验室开放基金（XNZW17C04）；湖南科技学院科研项目（17XKY009）；湖南科技学院大学生研究性学习和创新性实验计划项目（湘科院教发[2017]24 号）；湖南科技学院生物工程应用特色学科资助
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