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投稿时间：2018-10-18
投稿时间：2018-10-18
中文摘要: 探究高氧气调包装对影响鲜切菜心木质化的苯丙氨酸酶（phenylalaninammo－nialyase，PAL）的影响。采用高氧（氧含量大于90%）气调包装对鲜切菜心进行处理，以空气包装鲜切菜心作对照，置于25 ℃下贮藏，然后检测其木质素含量、PAL 活性，并通过反转录PCR（reverse transcription-PCR，RT-PCR）技术分析菜心PAL 基因转录表达差异。鲜切菜心木质素含量随着贮藏时间增长呈上升趋势，在贮藏5 d 后与对照组相比降低；PAL 活性及其基因转录表达均呈现先下降后升高的趋势，在3 d 时高氧组要低于对照组，5 d 后差异不大。高氧气调包装能一定程度上抑制PAL 基因的转录表达，降低PAL 的活性，减少木质素的含量。
Abstract:Explore the effect of high oxygen modified packaging （HOMP）on fresh cut cabbage lignified Phenylalaninammo-nialyase（PAL）.HOMP （oxygen＞90%）was used to pack the fresh-cut Chinese cabbage with air packaged as control.The treated cabbage was stored at 25 ℃，then the lignin content and PAL activity of the cabbage were detected every day during five days of storage，and the transcriptional expression of PAL gene by reverse transcription-polymerase chain reaction（RT-PCR）was also analyzed in chinese cabbage.The experimental results showd that，fresh-cut cabbage lignin content increased with storage time，with the HOMP lower than the control at the end of storage. The activity of PAL and its gene transcriptional expression showed that a tendency was found to decrease first and then increase.At 3 days，the HOMP was lower than the control，but there was not much difference at the end of storage. Therefore，the HOMP could inhibit the transcriptional expression of PAL gene，and reduce the activity of PAL and the lignin content of fresh-cut cabbage.
keywords: high oxygen modified packaging lignification phenylalaninammo-nialyase express the differences enzymatic activity
文章编号：201908005 中图分类号： 文献标志码：
基金项目:国家自然科学基金（31671912）；科技部“十二五”农村领域国家科技支撑计划（2015BAD16B0901）；广州市科技计划项目（201508020032）
| Author Name | Affiliation |
| LUO Zheng，GUO Yuan-yuan，CHEN Fei-ping，GONG Xiao-bo，CHEN Yu-long | Sericultural & Agri-food Research Institute GAAS，Guangzhou 510610，Guangdong，China |
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