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投稿时间：2018-07-17
投稿时间：2018-07-17
中文摘要: 建立鱼肉中的孔雀石绿、结晶紫及隐色代谢物的双柱在线净化（阳离子净化柱MCX一维净化，反相C8净化柱二维净化）-高效液相色谱-串联质谱测定方法。采用酸性乙腈提取样品，提取液直接上机分析，双柱在线净化系统净化，高效液相色谱-串联质谱测定。结果表明，鱼肉中孔雀石绿、结晶紫、隐色孔雀石绿、隐色结晶紫空白鱼肉3个不同水平的添加回收率（n=6）为76.8%～109.4%，相对标准偏差为2.0%～14.1%，4种药物定量限为0.5 μg/kg。
Abstract:A double column on-line purification was builded through combining the first purification of MCX column and the second purification of C8 column to determine the residues of malachite green，crystal ciolet and their leuco metabolites in fish by high performance liquid chromatography-tandem mass spectrometry.The samples were extracted with acid acetonitrile.The extract was directly injected into double column on-line purification system.The results showed that the average recoveries of three levels were from 76.8%to 109.4%，and the relative standard deviation ranged from 2.0%to 14.1%.The detection limits of the four drugs were 0.5 μg/kg.
keywords: double column on-line purification clean-up fish high performance liquid chromatographytandem mass spectrometry malachite green crystal violet leuco metabolites
文章编号：201905035 中图分类号： 文献标志码：
基金项目:国家质量监督检验检疫总局科技计划项目（2016IK251）；国家重点研发计划（2017YFF0210201）
| Author Name | Affiliation |
| ZHAO Kong-xiang，CHEN Xu-yan，LIU Yang，MI Jie-bo，ZHANG Hua | Animal&Plant and Food Inspection Center of Tianjin Custom，Tianjin 300456，China |
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