Abstract:The effects of ginseng water-soluble total protein on the damage of SH-SY5Y induced by amyloid β in human neuroblastoma were studied.In vitro model of Alzheimer's disease was established by treatment of SH-SY5Y cells with 25 μmol/L aging Aβ25-35.SH-SY5Y cells were pre-protected with ginseng water-soluble total protein(6.25，12.5，25 μg/mL)for 12 h.25 μm/L of Aβ25-35was added for 24 h.Cell viability was detected by CCK-8 method.Flow cytometry for apoptosis，reactive oxygen species(ROS)level and mitochondrial membrane potential were detected by flow cytometry.Ultraviolet spectrophotometric determination of adenosine 5'-triphosphate(ATP)and malondialdehyde(MDA)，superoxide dismutase(SOD)activity.Western blotting was used to detect cytochrome C release and apoptosis-related protein B-cell lymphoma-2 (Bcl-2)and Bcl-2 associated X protein(Bax)expression.The results showed that the cell viability was significantly decreased and the degree of apoptosis was significantly increased in SH-SY5Y cells induced by Aβ25-35(P＜0.0001).Different concentrations of ginseng total protein could reduce the decrease of cell viability and the degree of apoptosis induced by Aβ25-35，and it was concentration dependent.Pretreatment with ginseng total protein could reduce the increase of mitochondrial superoxide induced by Aβ25-35，inhibit the production of ROS，and significantly inhibit the decrease of mitochondrial membrane potential(MMP)induced by Aβ25-35injury，and decrease in the level of adenosine-triphosphate(ATP).Ginseng total protein inhibited the release of Cytochrome C to cytoplasm induced by Aβ25-35，down-regulated Bax expression and up-regulated Bcl-2 expression.