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食品研究与开发:2017,38(7):38-44
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芍药花多酚的纯化及其抗氧化活性研究
俞憬1,陈冠林2,杨璐齐1,王煜坤1,高永清1,傅南琳3,*
(1. 广东药科大学 食品科学学院,广东 中山 528458;2. 佛山市职业病防治所,广东 佛山 528000;3. 广东药科大学附属第一医院,广东 广州 510008)
Study on Purification and Antioxidant Activity of Polyphenols from the Flower of Paeonia lactiflora Palla
YU Jing1, CHEN Guan-lin2, YANG Lu-qi1, WANG Yu-kun1, GAO Yong-qing1, FU Nan-lin3,*
(1. School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, Guangdong, China;2. Foshan Institute of Occupational Disease Prevention and Control, Foshan 528000,Guangdong, China;3. The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510008, Guangdong, China)
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投稿时间:2016-07-26    
中文摘要: 研究大孔吸附树脂纯化芍药花多酚的条件和纯化后多酚的抗氧化能力。采用静态试验和动态试验确定纯化条 件,计算纯化后多酚的含量。在供试的 9 种大孔吸附树脂中,HPD100 树脂的吸附和洗脱效果最好。在室温下,以 50 %乙 醇为洗脱剂, 吸附流速和洗脱流速均为 10 mL/min 时, 纯化的芍药花多酚含量为 721.72 mg GAE/g DW, 是未纯化前的1.57 倍。通过 DPPH、FRAP 和 ABTS 三种方法测得纯化后芍药花多酚的抗氧化能力是未纯化前粗提物的 1.35、1.44、1.62倍。
中文关键词: 纯化;抗氧化;多酚;芍药花;大孔树脂
Abstract:Using the macroporous adsorptive resins, the purification conditions for total phenolic content(TPC) from the flower of Paeonia lactiflora Palla and the antioxidant capacity of the TPC were studied after purification. Static and dynamic experimental methods were performed to determine the purification conditions, and the con- tents of the purified TPC were calculated. Among the 9 macroporous resins tested, HPD100 resin was the best in adsorption effect and desorption effect. At room temperature, when 50 % of ethanol was used as desorption solu- tion, adsorption and desorption flow velocities were both 10 mL/min, the content of the TPC purified from the flower of Paeonia lactiflora Palla was 721.72 mg GAE/g dry weight, and it was 1.57 times higher compared with that before purification. The antioxidant activities of purified extracts measured by 2,2-diphenyl-1-picrylhy- drazyl radical scavenging activity assay(DPPH), ferric reducing antioxidant power assay(FRAP), and ABTS+ radical scavenging activity (ABTS)assay were 1.35, 1.44 ,1.62 times higher than those of crude extracts before purification, respectively.
keywords: purification; antioxidant; polyphenol; Paeonia lactiflora Palla; macroporous resins
文章编号:201707009     中图分类号:10.3969/j.issn.1005-6521.2017.07.009    文献标志码:A
基金项目:广东省医学科学技术研究基金项目(A2015617);中医药 强省建设专项资金第二批名中医师承项目(粤中医办函[2015]93 号) 作者简介:俞憬(1992—),男(汉),在读硕士,研究方向:营养与 健康。
作者单位
俞憬1,陈冠林2,杨璐齐1,王煜坤1,高永清1,傅南琳3,* 1. 广东药科大学 食品科学学院,广东 中山 528458;2. 佛山市职业病防治所,广东 佛山 528000;3. 广东药科大学附属第一医院,广东 广州 510008 
Author NameAffiliation
YU Jing1, CHEN Guan-lin2, YANG Lu-qi1, WANG Yu-kun1, GAO Yong-qing1, FU Nan-lin3,* 1. School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, Guangdong, China;2. Foshan Institute of Occupational Disease Prevention and Control, Foshan 528000,Guangdong, China;3. The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510008, Guangdong, China 
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